Regulatory

Part:BBa_J100404:Design

Designed by: Tatianna Travieso, Caroline Willis   Group: Campbell M Lab   (2018-06-14)


T7 Promoter with Gal4 Binding Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The paper referenced earlier discussed studies that found an "initiation domain" and a "binding domain" from -12 to +5, both of which were essential for successful transcription and translation. We introduced the binding site within the "dispensable region" from -12 to -17.


Source

"T7 promoter contacts essential for promoter activity in vivo" by Richard A. Iketa et al. (1992), https://www.ncbi.nlm.nih.gov/pubmed/1598210 Consensus sequence, https://parts.igem.org/Promoters/Catalog/T7

References